Compromised stability of DNA methylation and transposon immobilization in mosaic Arabidopsis epigenomes.
- Reinders J Department of Plant Biology, University of Geneva, Sciences III, Geneva, Switzerland. jon.reinders@unige.ch
- Wulff BB
- Mirouze M
- Marí-Ordóñez A
- Dapp M
- Rozhon W
- Bucher E
- Theiler G
- Paszkowski J
- 2009-04-25
Published in:
- Genes & development. - 2009
Arabidopsis
Arabidopsis Proteins
Chromosome Mapping
Chromosomes, Plant
DNA (Cytosine-5-)-Methyltransferases
DNA Methylation
DNA Transposable Elements
Epigenesis, Genetic
Genes, Plant
Genome, Plant
Genomic Instability
Inbreeding
Mosaicism
Phenotype
Recombination, Genetic
English
Transgenerational epigenetic inheritance has been defined by the study of relatively few loci. We examined a population of recombinant inbred lines with epigenetically mosaic chromosomes consisting of wild-type and CG methylation-depleted segments (epiRILs). Surprisingly, transposons that were immobile in the parental lines displayed stochastic movement in 28% of the epiRILs. Although analysis after eight generations of inbreeding, supported by genome-wide DNA methylation profiling, identified recombined parental chromosomal segments, these were interspersed with unexpectedly high frequencies of nonparental methylation polymorphism. Hence, epigenetic inheritance in hybrids derived from parents with divergent epigenomes permits long-lasting epi-allelic interactions that violate Mendelian expectations. Such persistently "unstable" epigenetic states complicate linkage-based epigenomic mapping. Thus, future epigenomic analyses should consider possible genetic instabilities and alternative mapping strategies.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1101/gad.524609
- PMID 19390088
- Persistent URL
- https://sonar.ch/global/documents/21220
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