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A Microfluidic Biodisplay.
Journal article

A Microfluidic Biodisplay.

  • Volpetti F Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne , 1015 Lausanne, Switzerland.
  • Petrova E Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne , 1015 Lausanne, Switzerland.
  • Maerkl SJ Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne , 1015 Lausanne, Switzerland.
  • 2017-08-04
Published in:
  • ACS synthetic biology. - 2017
arsenic
biodisplay
continuous bacterial culturing
environmental sensor
heavy metal sensor
microfluidics
Bacillus subtilis
Cell Engineering
Escherichia coli
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques
English Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves as an interface between an engineered cellular system, environment, and user. We created a biodisplay consisting of 768 individually programmable biopixels and demonstrated that it can perform multiplexed, continuous sampling. The biodisplay detected 10 μg/L sodium-arsenite in tap water using a research grade fluorescent microscope, and reported arsenic contamination down to 20 μg/L with an easy to interpret "skull and crossbones" symbol detectable with a low-cost USB microscope or by eye. The biodisplay was designed to prevent release of chemical or biological material to avoid environmental contamination. The microfluidic biodisplay thus provides a practical solution for the deployment and application of engineered cellular systems.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/215208
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