Journal article
Organ specific gene expression in the regenerating tail of Macrostomum lignano.
- Lengerer B Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. Electronic address: birgit.lengerer@uibk.ac.at.
- Wunderer J Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. Electronic address: julia.wunderer@uibk.ac.at.
- Pjeta R Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. Electronic address: robert.pjeta@uibk.ac.at.
- Carta G Division of Physiology, Medical University of Innsbruck, Schöpfstraße 41/EG, A-6020 Innsbruck, Austria. Electronic address: giada.carta@i-med.ac.at.
- Kao D Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom. Electronic address: damian.kao@gmail.com.
- Aboobaker A Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, United Kingdom. Electronic address: aziz.aboobaker@zoo.ox.ac.uk.
- Beisel C Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058 Basel, Switzerland. Electronic address: christian.beisel@bsse.ethz.ch.
- Berezikov E European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, A. Deusinglaan 1, NL-9713 AV Groningen, The Netherlands. Electronic address: e.berezikov@umcg.nl.
- Salvenmoser W Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. Electronic address: willi.salvenmoser@uibk.ac.at.
- Ladurner P Institute of Zoology and Center of Molecular Bioscience Innsbruck, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. Electronic address: peter.ladurner@uibk.ac.at.
- 2017-08-01
Published in:
- Developmental biology. - 2018
Differentiation
Male copulatory apparatus
Stylet
in situ hybridization
Animals
Gene Expression Regulation
Genes, Helminth
Helminth Proteins
Hermaphroditic Organisms
In Situ Hybridization
Microvilli
Organ Specificity
Platyhelminths
RNA Interference
RNA, Helminth
RNA, Messenger
Regeneration
Tail
Transcriptome
Wound Healing
English
Temporal and spatial characterization of gene expression is a prerequisite for the understanding of cell-, tissue-, and organ-differentiation. In a multifaceted approach to investigate gene expression in the tail plate of the free-living marine flatworm Macrostomum lignano, we performed a posterior-region-specific in situ hybridization screen, RNA sequencing (RNA-seq) of regenerating animals, and functional analyses of selected tail-specific genes. The in situ screen revealed transcripts expressed in the antrum, cement glands, adhesive organs, prostate glands, rhabdite glands, and other tissues. Next we used RNA-seq to characterize temporal expression in the regenerating tail plate revealing a time restricted onset of both adhesive organs and copulatory apparatus regeneration. In addition, we identified three novel previously unannotated genes solely expressed in the regenerating stylet. RNA interference showed that these genes are required for the formation of not only the stylet but the whole male copulatory apparatus. RNAi treated animals lacked the stylet, vesicula granulorum, seminal vesicle, false seminal vesicle, and prostate glands, while the other tissues of the tail plate, such as adhesive organs regenerated normally. In summary, our findings provide a large resource of expression data during homeostasis and regeneration of the morphologically complex tail regeneration and pave the way for a better understanding of organogenesis in M. lignano.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1016/j.ydbio.2017.07.021
- PMID 28757111
- Persistent URL
- https://sonar.ch/global/documents/223395
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