Back
Protein precipitation for the analysis of a drug cocktail in plasma by LC-ESI-MS.
Journal article

Protein precipitation for the analysis of a drug cocktail in plasma by LC-ESI-MS.

  • Souverain S Laboratory of Analytical Pharmaceutical Chemistry, School of Pharmacy, University of Geneva, 20 bvd d'Yvoy, 1211 Geneva 4, Switzerland.
  • Rudaz S
  • Veuthey JL
  • 2004-06-15
Published in:
  • Journal of pharmaceutical and biomedical analysis. - 2004
Blood Proteins
Chemical Precipitation
Chromatography, Liquid
Humans
Spectrometry, Mass, Electrospray Ionization
English Three protein precipitation (PP) procedures with acetonitrile (ACN), perchloric acid (PA) and trichloroacetic acid (TCA) were investigated for the analysis of a drug cocktail from human plasma samples containing three pharmaceutical compounds and their primary metabolites. For this purpose, a capillary liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) method was developed for the simultaneous analysis of the six tested compounds in less than 6 min. Matrix effect was tested for each PP procedure by means of a post-column infusion system. The three PP techniques were found effective in removing proteins from human plasma and were fully compatible with capillary LC-ESI-MS analysis. However, with acid precipitations, low analyte recovery and a high variability, probably due to analyte coprecipitation, were obtained. Finally, ACN was found to be the most effective PP technique with a recovery higher than 80% and CV inferior to 6%.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/230495
Statistics
Document views: 53 File downloads: