Back
Analysis of potential household transmission events of tuberculosis in the city of Belem, Brazil.
Journal article

Analysis of potential household transmission events of tuberculosis in the city of Belem, Brazil.

  • Conceição EC Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Goés, Rio de Janeiro, Brazil. Electronic address: emilyncosta@gmail.com.
  • Guimarães AEDS Universidade do Estado do Pará, Programa de Pós-graduação em Biologia Parasitária na Amazônia, Belém, Brazil.
  • Lopes ML Instituto Evandro Chagas, Seção de Bacteriologia e Micologia, Belém, Brazil.
  • Furlaneto IP Universidade do Estado do Pará, Programa de Pós-graduação em Biologia Parasitária na Amazônia, Belém, Brazil.
  • Rodrigues YC Universidade do Estado do Pará, Programa de Pós-graduação em Biologia Parasitária na Amazônia, Belém, Brazil.
  • da Conceição ML Universidade do Estado do Pará, Programa de Pós-graduação em Biologia Parasitária na Amazônia, Belém, Brazil.
  • Barros WA Universidade Federal do Pará, Hospital Universitário João de Barros Barreto, Belém, Brazil.
  • Cardoso NC Universidade Federal do Pará, Hospital Universitário João de Barros Barreto, Belém, Brazil.
  • Sharma A University of Delhi, Delhi College of Engineering, New Delhi, India.
  • Lima LNGC Universidade do Estado do Pará, Programa de Pós-graduação em Biologia Parasitária na Amazônia, Belém, Brazil; Instituto Evandro Chagas, Seção de Bacteriologia e Micologia, Belém, Brazil.
  • Gomes HM Instituto Oswaldo Cruz - FIOCRUZ, Laboratório de Biologia Molecular Aplicada as Micobactérias, Rio de Janeiro, Brazil.
  • Duarte RS Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Goés, Rio de Janeiro, Brazil.
  • Frota C Universidade Federal do Ceará, Departamento de Patologia e Medicina Legal, Fortaleza, Ceará, Brazil.
  • Rutaihwa LK Swiss Tropical & Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
  • Gagneux S Swiss Tropical & Public Health Institute, Basel, Switzerland; University of Basel, Basel, Switzerland.
  • Suffys PN Instituto Oswaldo Cruz - FIOCRUZ, Laboratório de Biologia Molecular Aplicada as Micobactérias, Rio de Janeiro, Brazil. Electronic address: psuffys@ioc.fiocruz.br.
  • Lima KVB Universidade do Estado do Pará, Programa de Pós-graduação em Biologia Parasitária na Amazônia, Belém, Brazil; Instituto Evandro Chagas, Seção de Bacteriologia e Micologia, Belém, Brazil. Electronic address: karlalima@iec.pa.gov.br.
Show more…
  • 2018-12-06
Published in:
  • Tuberculosis (Edinburgh, Scotland). - 2018
Genotyping
Household contacts
Molecular epidemiology
Multi-drug resistance
Tuberculosis
Whole genome sequencing
Adolescent
Adult
Aged
Antitubercular Agents
Bacteriological Techniques
Brazil
Child
Contact Tracing
DNA, Bacterial
Drug Resistance, Multiple, Bacterial
Family Characteristics
Female
Genotype
Humans
Interspersed Repetitive Sequences
Male
Microbial Sensitivity Tests
Middle Aged
Molecular Epidemiology
Mycobacterium tuberculosis
Phenotype
Predictive Value of Tests
Reproducibility of Results
Tandem Repeat Sequences
Tuberculosis
Tuberculosis, Multidrug-Resistant
Whole Genome Sequencing
Young Adult
English Tuberculosis (TB) is an infectious disease with a higher risk for infection and disease among household contacts (HHC). Here, we report a molecular epidemiology-based approach to study disease transmission and the genetic characteristics of Mycobacterium tuberculosis (Mtb) strains among HHC in the city of Belem, the capital of the state of Para in north Brazil. The study included 63 TB patients belonging to 26 HHC groups (HHC1 to HHC26). Spoligotyping and 24-loci Mycobacterial Interspersed Repetitive Unit - Variable Number of Tandem Repeat (MIRU-VNTR) revealed indistinguishable bacterial genotypes among 26 patients in 14 (53.8%) HHC groups. Drug susceptibility testing (DST) revealed that 45 (71.4%) of the Mtb isolates were multidrug resistant. The major cluster composed of isolates from five HHCs and on three of these, whole genome sequencing (WGS) was performed confirming their high genetic similarity. These results pinpoint the need for improved vigilance for TB control in households in the city of Belém. When comparing WGS versus phenotypic resistance detection methods as DST and Minimum Inhibitory Concentration (MIC) our data suggest that depending on the colonies selection, results may present variation.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/232449
Statistics
Document views: 22 File downloads: