Journal article
Regenerative properties of collagenated porcine bone grafts in human maxilla: demonstrative study of the kinetics by synchrotron radiation microtomography and light microscopy.
- Giuliani A Department of Clinical Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131, Ancona, Italy. a.giuliani@univpm.it.
- Iezzi G Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy.
- Mazzoni S Department of Clinical Sciences, Polytechnic University of Marche, Via Brecce Bianche, 60131, Ancona, Italy.
- Piattelli A Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy.
- Perrotti V Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, Via dei Vestini 31, 66100, Chieti Scalo, CH, Italy.
- Barone A Department of Surgery, Unit of Oral Surgery and Implantology, School of Dental Medicine University of Geneve, Geneve, Switzerland.
- 2017-06-04
Published in:
- Clinical oral investigations. - 2018
Biomaterial
Collagenated porcine bone
Histology
Post-extraction sockets
Synchrotron radiation microtomography
Adult
Aged
Alveolar Ridge Augmentation
Animals
Bone Remodeling
Bone Substitutes
Bone Transplantation
Female
Heterografts
Humans
Imaging, Three-Dimensional
Male
Maxilla
Middle Aged
Swine
Synchrotrons
Tooth Extraction
Tooth Socket
Treatment Outcome
Wound Healing
X-Ray Microtomography
English
OBJECTIVES
Recently, it has been reported that heterologous biomaterials, where the collagen matrix is preserved, seem to facilitate blood clotting and the subsequent invasion of repairing and regenerative cells. This study aimed at evaluating the healing of human extraction sockets grafted with a collagenated cortico-cancellous porcine bone (mp3®, OsteoBiol®, Tecnoss®, Giaveno, Italy) by synchrotron radiation X-ray microtomography (microCT) and histology in order to have a 3D quantitative characterization over time.
MATERIALS AND METHODS
Ridge preservation with porcine bone and collagen membrane was performed on 21 patients using a flapless approach and a secondary soft tissue closure. At the time of implant placement, six bone samples were harvested, two in the 3-month group, two in the 6-month group, one in the 12-month group post-surgery, and one spontaneously healed control, and evaluated by microCT and histology.
RESULTS
MicroCT revealed that in the grafted sites there were a greater number of trabeculae, although they appeared thinner than in the control sites; these trabeculae homogenously filled the defects, suggesting an improved strength of the socket. Histology showed that over time, the amount of biomaterial decreased and the newly formed bone increased, while less dense bone with wider marrow spaces was detected in the control sample, supporting synchrotron findings.
CONCLUSIONS
The morphometric data converge to indicate the suitability of porcine bone for the preservation of post-extraction sockets.
CLINICAL RELEVANCE
The use of mp3 is encouraged to preserve and heal sockets.
Recently, it has been reported that heterologous biomaterials, where the collagen matrix is preserved, seem to facilitate blood clotting and the subsequent invasion of repairing and regenerative cells. This study aimed at evaluating the healing of human extraction sockets grafted with a collagenated cortico-cancellous porcine bone (mp3®, OsteoBiol®, Tecnoss®, Giaveno, Italy) by synchrotron radiation X-ray microtomography (microCT) and histology in order to have a 3D quantitative characterization over time.
MATERIALS AND METHODS
Ridge preservation with porcine bone and collagen membrane was performed on 21 patients using a flapless approach and a secondary soft tissue closure. At the time of implant placement, six bone samples were harvested, two in the 3-month group, two in the 6-month group, one in the 12-month group post-surgery, and one spontaneously healed control, and evaluated by microCT and histology.
RESULTS
MicroCT revealed that in the grafted sites there were a greater number of trabeculae, although they appeared thinner than in the control sites; these trabeculae homogenously filled the defects, suggesting an improved strength of the socket. Histology showed that over time, the amount of biomaterial decreased and the newly formed bone increased, while less dense bone with wider marrow spaces was detected in the control sample, supporting synchrotron findings.
CONCLUSIONS
The morphometric data converge to indicate the suitability of porcine bone for the preservation of post-extraction sockets.
CLINICAL RELEVANCE
The use of mp3 is encouraged to preserve and heal sockets.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1007/s00784-017-2139-6
- PMID 28577053
- Persistent URL
- https://sonar.ch/global/documents/232794
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