A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection.

van der Mee-Marquet NL; Bénéjat L; Diene SM; Lemaignen A; Gaïa N; Smet A; Haesebrouck F; Cherkaoui A; Ducournau A; Lacomme S; Gontier E; Bernard L; Mégraud F; Goudeau A; Lehours P; François P

doi:10.3389/fmicb.2017.02533

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Journal article

A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection.

van der Mee-Marquet NL Service de Bactériologie, Virologie et Hygiène, Hôpital Trousseau, Réseau des Hygiénistes du Centre, CPIAS Centre Val de Loire, Centre Hospitalier Régional Universitaire, and UMR 1282 Infectiologie Santé Publique, Université François-Rabelais, Tours, France.
Bénéjat L Laboratoire de Bactériologie, Centre National de Référence des Campylobacters et des Hélicobacters, Bordeaux, France.
Diene SM Faculté de Médecine et de Pharmacie, URMITE, Aix-Marseille Université, UMR 63, Centre National de la Recherche Scientifique 7278, IRD 198, Institut National de la Santé et de la Recherche Médicale, IHU-Méditerranée Infection, Marseille, France.
Lemaignen A Service de Médecine Interne et des Maladies Infectieuses, Centre Hospitalier Régional Universitaire, Hôpital Bretonneau, Tours, France.
Gaïa N Genomic Research Laboratory, University Hospital of Geneva, Geneva, Switzerland.
Smet A Laboratory Experimental Medicine and Pediatrics, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium.
Haesebrouck F Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Cherkaoui A Bacteriology Laboratory, Division of Laboratory Medicine, Geneva University Hospitals, Geneva, Switzerland.
Ducournau A Laboratoire de Bactériologie, Centre National de Référence des Campylobacters et des Hélicobacters, Bordeaux, France.
Lacomme S Bordeaux Imaging Center, Imagerie Electronique, UMS 3420 Centre National de la Recherche Scientifique US4 Institut National de la Santé et de la Recherche Médicale Université de Bordeaux, Bordeaux, France.
Gontier E Bordeaux Imaging Center, Imagerie Electronique, UMS 3420 Centre National de la Recherche Scientifique US4 Institut National de la Santé et de la Recherche Médicale Université de Bordeaux, Bordeaux, France.
Bernard L Service de Médecine Interne et des Maladies Infectieuses, Centre Hospitalier Régional Universitaire, Hôpital Bretonneau, Tours, France.
Mégraud F Laboratoire de Bactériologie, Centre National de Référence des Campylobacters et des Hélicobacters, Bordeaux, France.
Goudeau A Service de Bactériologie, Virologie et Hygiène, Hôpital Trousseau, Réseau des Hygiénistes du Centre, CPIAS Centre Val de Loire, Centre Hospitalier Régional Universitaire, and UMR 1282 Infectiologie Santé Publique, Université François-Rabelais, Tours, France.
Lehours P Laboratoire de Bactériologie, Centre National de Référence des Campylobacters et des Hélicobacters, Bordeaux, France.
François P Genomic Research Laboratory, University Hospital of Geneva, Geneva, Switzerland.

2018-01-13

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Frontiers in microbiology. - 2017

English We isolated from aerobic and anaerobic blood culture bottles from a febrile patient, a Helicobacter-like Gram negative, rod-shaped bacterium that MALDI-TOF MS failed to identify. Blood agar cultures incubated in a microaerobic atmosphere revealed a motile Gram negative rod, which was oxidase, catalase, nitrate reductase, esterase, and alkaline phosphatase positive. It grew at 42°C with no detectable urease activity. Antimicrobial susceptibility testing showed that the organism was susceptible to beta-lactams, gentamicin, erythromycin, and tetracycline but resistant to ciprofloxacin. Electronic microscopy analysis revealed a 3 × 0.5 μm curved rod bacterium harboring two sheathed amphitrichous flagella. Whole genome sequencing revealed a genome 1,708,265 base-pairs long with a GC content of 37.80% and a total of 1,697 coding sequences. The genomic analyses using the nucleotide sequences of the 16S rRNA gene, hsp60 and gyrB genes, as well as the GyrA protein sequence, and the results of Average Nucleotide Identity and in silico DNA-DNA hybridization suggest evidence for a novel Helicobacter species close to Helicobacter equorum and belonging to the group of enterohepatic Helicobacter species. As soon as the particular peptide mass fingerprint of this pathogen is added to the spectral databases, MALDI-TOF MS technology will improve its identification from clinical specimens, especially in case of "sterile infection". We propose to associate the present strain with the Latin name of the place of isolation; Caesarodunum (Tours, France) and suggest "Helicobacter caesarodunensis" for further description of this new bacterium.

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