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Repair of DNA Damage Induced by the Cytidine Analog Zebularine Requires ATR and ATM in Arabidopsis.

  • Liu CH Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
  • Finke A Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
  • Díaz M Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
  • Rozhon W Biotechnology of Horticultural Crops, Technische Universität München, 85354 Freising, Germany.
  • Poppenberger B Biotechnology of Horticultural Crops, Technische Universität München, 85354 Freising, Germany.
  • Baubec T University of Zürich, 8057 Zürich, Switzerland.
  • Pecinka A Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany pecinka@mpipz.mpg.de.
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  • 2015-05-30
Published in:
  • The Plant cell. - 2015
Arabidopsis
Arabidopsis Proteins
Ataxia Telangiectasia Mutated Proteins
Cell Cycle
Cytidine
DNA Damage
DNA Repair
DNA Replication
English DNA damage repair is an essential cellular mechanism that maintains genome stability. Here, we show that the nonmethylable cytidine analog zebularine induces a DNA damage response in Arabidopsis thaliana, independent of changes in DNA methylation. In contrast to genotoxic agents that induce damage in a cell cycle stage-independent manner, zebularine induces damage specifically during strand synthesis in DNA replication. The signaling of this damage is mediated by additive activity of ATAXIA TELANGIECTASIA MUTATED AND RAD3-RELATED and ATAXIA TELANGIECTASIA MUTATED kinases, which cause postreplicative cell cycle arrest and increased endoreplication. The repair requires a functional STRUCTURAL MAINTENANCE OF CHROMOSOMES5 (SMC5)-SMC6 complex and is accomplished predominantly by synthesis-dependent strand-annealing homologous recombination. Here, we provide insight into the response mechanism for coping with the genotoxic effects of zebularine and identify several components of the zebularine-induced DNA damage repair pathway.
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  • English
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bronze
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https://sonar.ch/global/documents/235777
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