Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes.

Lescat M; Poirel L; Nordmann P

doi:10.1016/j.diagmicrobio.2018.04.010

Back

Journal article

Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes.

Lescat M Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Université Paris Nord, Sorbonne Paris Cité, Paris, France; AP-HP, Hôpitaux Universitaires Paris Seine Saint-Denis, Bondy, France.
Poirel L Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland. Electronic address: laurent.poirel@unifr.ch.
Nordmann P Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland; INSERM European Unit (LEA, IAME), Paris, France; Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland; University of Lausanne and University Hospital Center, Lausanne, Switzerland.

2018-09-18

Published in:

Diagnostic microbiology and infectious disease. - 2018

Multiplex Polymerase Chain Reaction

Transferases (Other Substituted Phosphate Groups)

English A rapid (total time <2 h) and reliable multiplex polymerase chain reaction for screening of mcr-1 to mcr-5 genes conferring resistance to colistin has been developed. This technique has been tested on a collection of isolates previously identified as bearing mcr-1, mcr-2, and mcr-like genes and had a sensitivity and a specificity of 100%. Using this method, we were also able to identify a single isolate possessing both mcr-1 and mcr-5 genes.

Language