Nanoscale bioactive glass activates osteoclastic differentiation of RAW 264.7 cells.
- Detsch R Department of Materials Science & Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
- Rübner M Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
- Strissel PL Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
- Mohn D Institute for Chemical & Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland.
- Strasser E Department of Transfusion Medicine & Haemostaseology, University-Clinic Erlangen, Krankenhausstraße 12, 91054 Erlangen, Germany.
- Stark WJ Institute for Chemical & Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, 8093 Zurich, Switzerland.
- Strick R Department of Gynaecology & Obstetrics, Laboratory for Molecular Medicine, Friedrich-Alexander University Erlangen-Nürnberg (FAU), University-Clinic Erlangen, Universitätsstraße 21-23, 91054 Erlangen, Germany.
- Boccaccini AR Department of Materials Science & Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
- 2016-04-20
Published in:
- Nanomedicine (London, England). - 2016
bioactive glass
biomaterials
bone tissue engineering
nanoparticles
osteoclasts
Animals
Biocompatible Materials
Cell Differentiation
Cell Proliferation
Ceramics
Gene Expression Regulation, Developmental
Glass
Humans
Mice
Monocytes
Osteoclasts
RAW 264.7 Cells
English
BACKGROUND
There is limited knowledge regarding differentiation of osteoclasts in the presence of nanoscale bioactive glass (nBG). This investigation examined increasing concentrations of 45S5 nBG and their influence on osteoclast differentiation.
MATERIALS & METHODS
Different concentrations of 45S5 nBG were cultured up to 14 days with the murine RAW264.7 cell line and human primary monocytes cultured with M-CSF and RANKL.
RESULTS
Culturing cells for 14 days with 500 μg/ml nBG showed a viability of 100%; however DNA synthesis was reduced, supporting differentiation into osteoclast-like cells. Using RAW cells, activation of nine genes, including cell fusion genes, occurred in an nBG concentration dependent manner. Low concentrations of nBG increased expression of genes involved in commitment to cell fusion, whereas high concentrations increased gene expression supporting osteoclast-like differentiation.
CONCLUSION
nBG enhances both RAW264.7 and human osteoclast differentiation. nBG controlled gene expression in a concentration dependent manner could reflect normal regulation during bone growth.
There is limited knowledge regarding differentiation of osteoclasts in the presence of nanoscale bioactive glass (nBG). This investigation examined increasing concentrations of 45S5 nBG and their influence on osteoclast differentiation.
MATERIALS & METHODS
Different concentrations of 45S5 nBG were cultured up to 14 days with the murine RAW264.7 cell line and human primary monocytes cultured with M-CSF and RANKL.
RESULTS
Culturing cells for 14 days with 500 μg/ml nBG showed a viability of 100%; however DNA synthesis was reduced, supporting differentiation into osteoclast-like cells. Using RAW cells, activation of nine genes, including cell fusion genes, occurred in an nBG concentration dependent manner. Low concentrations of nBG increased expression of genes involved in commitment to cell fusion, whereas high concentrations increased gene expression supporting osteoclast-like differentiation.
CONCLUSION
nBG enhances both RAW264.7 and human osteoclast differentiation. nBG controlled gene expression in a concentration dependent manner could reflect normal regulation during bone growth.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.2217/nnm.16.20
- PMID 27092984
- Persistent URL
- https://sonar.ch/global/documents/265743
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