In Vitro Study of ISApl1-Mediated Mobilization of the Colistin Resistance Gene mcr-1.

Poirel L; Kieffer N; Nordmann P

doi:10.1128/AAC.00127-17

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Journal article

In Vitro Study of ISApl1-Mediated Mobilization of the Colistin Resistance Gene mcr-1.

Poirel L Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland laurent.poirel@unifr.ch.
Kieffer N Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland.
Nordmann P Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland.

2017-04-19

Published in:

Antimicrobial agents and chemotherapy. - 2017

English The plasmid-mediated mcr-1 gene encodes a phosphoethanolamine transferase that confers resistance to polymyxins. The mcr-1 gene is associated with insertion sequence ISApl1 (IS30 family). In vitro mobilization assays demonstrated the functionality of the composite transposon structure ISApl1-mcr-1-ISApl1 Transposition generated a 2-bp duplication and occurred in AT-rich DNA regions. This is the first report demonstrating the mobility of the mcr-1 gene by transposition.

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