Identification, molecular characterization and prevalence of a novel cytorhabdovirus infecting zucchini crops in Greece.

Orfanidou CG; Beta C; Reynard JS; Tsiolakis G; Katis NI; Maliogka VI

doi:10.1016/j.virusres.2020.198095

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Identification, molecular characterization and prevalence of a novel cytorhabdovirus infecting zucchini crops in Greece.

Orfanidou CG Laboratory of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Beta C Laboratory of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Reynard JS Laboratory of Virology, Agroscope, Route de Duillier 50, 1260, Nyon, Switzerland.
Tsiolakis G Laboratory of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Katis NI Laboratory of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.
Maliogka VI Laboratory of Plant Pathology, Faculty of Agriculture, Forestry and Natural Environment, School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece. Electronic address: vmaliogk@agro.auth.gr.

2020-08-01

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Virus research. - 2020

English A new cytorhabdovirus was identified in zucchini (Cucurbita pepo) in Greece with the aid of high-throughput sequencing technology. The negative-sense, single-stranded genomic RNA of the new virus was determined and includes seven open reading frames in the order 3'-N-P-P3-P4-M-G-L-5' in the antigenomic orientation. Typical rhabdovirus-like particles were observed in infected leaf material. Comparative sequence analysis and phylogenetic reconstructions suggested that the described virus is a new member of the genus Cytorhabdovirus, and it was tentatively named cucurbit cytorhabdovirus 1 (CuCV1). To our knowledge CuCV1 is the first cytorhabdovirus infecting cucurbits in nature. Our surveys indicated that it occurs in a percentage of 36.7 % in zucchini crops in Greece.

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