Propagation of the virus of porcine epidemic diarrhea in cell culture.
- 1988-11-01
Published in:
- Journal of clinical microbiology. - 1988
Animals
Coronaviridae
Culture Techniques
Fluorescent Antibody Technique
Microscopy, Electron
Transmissible gastroenteritis virus
Vero Cells
Viral Plaque Assay
English
Porcine epidemic diarrhea virus (PEDV) was adapted to serial propagation in Vero cell cultures by adding trypsin to the medium. PEDV-infected cells showed a distinct cytoplasmic fluorescence when examined by a fluorescent-antibody-staining technique. Cytopathic effects, such as vacuolation, formation of syncytia, and fusion of cells, were detected even at passage 1 of the PEDV in Vero cells. Once adapted, the virus induced numerous syncytia containing over 100 nuclei. From virus passage 5 on, all cells forming the monolayer were fused and totally destroyed within 24 h after inoculation. Cell culture-grown PEDV had typical coronavirus morphology when viewed by electron microscopy. Attempts to propagate PEDV in several primary and secondary fetal porcine cell cultures in the presence or absence of trypsin were unsuccessful.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1128/JCM.26.11.2235-2239.1988
- PMID 2853174
- Persistent URL
- https://sonar.ch/global/documents/30383
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