In-vitro study of ISApl1-mediated mobilization of the colistin resistance gene mcr-1

Poirel, Laurent; Kieffer, Nicolas; Nordmann, Patrice

doi:10.1128/AAC.00127-17

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Journal article

In-vitro study of ISApl1-mediated mobilization of the colistin resistance gene mcr-1

Université de Fribourg

Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland - French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland)
Kieffer, Nicolas Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland - French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland)
Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland - French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland) - University of Lausanne and University hospital Center, Lausanne, Switzerland

17.04.2017

Published in:

Antimicrobial Agents and Chemotherapy. - 2017, p. AAC.00127-17

English The plasmid-mediated mcr-1 gene encodes a phosphoethanolamine transferase conferring resistance to polymyxins. The mcr-1 gene is associated with insertion sequence ISApl1 (IS30 family). In-vitro mobilization assays demonstrated the functionality of the composite transposon structure ISApl1-mcr-1-ISApl1. Transposition generated a 2-bp duplication and occurred in AT-rich DNA regions. This is the first report demonstrating the mobility of the mcr-1 gene by transposition.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language