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Molecular parameters in melittin immunogenicity.
Journal article

Molecular parameters in melittin immunogenicity.

  • 1997-07-01
Published in:
  • Journal of peptide science : an official publication of the European Peptide Society. - 1997
Amino Acid Sequence
Animals
Cell Division
Cell Line
Clone Cells
Epitopes, T-Lymphocyte
Guinea Pigs
Humans
Melitten
Mice
Mice, Inbred BALB C
Molecular Sequence Data
T-Lymphocytes
English Based on immunogenicity studies, two T-cell epitopes in melittin were found to be functional in guinea pigs, one being centrally located, the other one residing in the C-terminal chain. In Balb/c mice only the central epitope was found to be active. A human T-cell clone was found by T-cell proliferation studies to employ strictly the C-terminal chain. Truncation of melittin peptides at the N-terminus did not markedly affect the capacity of guinea pigs to develop anti-IgG responses towards peptidic epitopes and towards a C-terminally attached haptenic group. Attachment of various substituents inside and outside the T-cell epitopic areas had no marked effect on antibody responses. In contrast, the substituents positioned within a T-cell epitope abolished T-cell proliferation. This difference between whole animal data and cellular in vitro responses is presently not understood.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/32764
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