Interactions between NPY and its receptor: assessment using ant-NPY antibodies.

Grouzmann E; Cressier F; Walker P; Hofbauer K; Waeber B; Brunner HR

doi:10.1016/0167-0115(94)90541-x

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Journal article

Interactions between NPY and its receptor: assessment using ant-NPY antibodies.

Grouzmann E Hypertension Division and Cardiovascular Research Group University Hospital, Lausanne, Switzerland.
Cressier F
Walker P
Hofbauer K
Waeber B
Brunner HR

1994-12-15

Published in:

Regulatory peptides. - 1994

English The present data show that monoclonal antibodies (NPY02, NPY03, NPY04, NPY05) directed against 4 distinct epitopes on NPY may have different actions on NPY binding and NPY-induced cellular responses. NPY02 and NPY05 recognize the 11-24 and 32-36 amidated form of NPY, respectively. These 2 antibodies block the binding of NPY to its receptor as well as the NPY-induced inhibition of cAMP accumulation caused in SK-N-MC cells by forskolin. NPY02 and NPY05 have also an inhibitory action on NPY-induced contraction of rabbit femoral arteries. NPY03 and NPY04 are directed against the 27-34 and 1-12 part of NPY, respectively. NPY03 and NPY04 inhibit the binding of NPY only at very high concentrations and have a weak effect on cAMP response to NPY. NPY02 and NPY05 might provide useful tools to study the effect of NPY in cellular systems and organ preparations.

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