Synthesis of enzymatically active D-7,8-dihydroneopterin-3'-triphosphate.

Zagalak B; Neuheiser F; Redweik U; Bosshard R; Leimbacher W

doi:10.1016/s0006-291x(88)80411-x

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Journal article

Synthesis of enzymatically active D-7,8-dihydroneopterin-3'-triphosphate.

Zagalak B Department of Pediatrics, University of Zurich, Switzerland.
Neuheiser F
Redweik U
Bosshard R
Leimbacher W

1988-05-16

Published in:

Biochemical and biophysical research communications. - 1988

English The first chemical synthesis of D-neopterin-3'-triphosphate and D-7,8-dihydroneopterin-3'-triphosphate is described. D-neopterin-3'-monophosphate was first 1'-2'-0-formylated with anhydrous formic acid, then activated with 1,1'-carbonyldiimidazole and phosphorylated with n-tributyl-ammonium pyrophosphate. The yield of 3'-NTP was 24%. D-7,8-dihydroneopterin-3'-triphosphate was obtained by chemical (hyposulfite) or catalytic (Pd:H2) reduction of 3'-NTP. Preparations from both reductions were fully active in two different enzymatic systems: synthesis of L-5,6,7,8-tetrahydrobiopterin and in the C-2'-epimerization reaction to L-7,8-dihydromonapterin-3'-triphosphate.

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