Back
Full-text
Journal article

Generation of a tightly regulated all-cis beta cell-specific tetracycline-inducible vector.

  • 2008-10-16
Published in:
  • BioTechniques. - 2008
Animals
COS Cells
Chlorocebus aethiops
GTPase-Activating Proteins
Gene Expression Regulation
Genetic Techniques
Genetic Vectors
Humans
Insulin-Secreting Cells
Mice
Organ Specificity
Plasmids
Response Elements
Tetracycline
English Ability to induce protein expression at will in a cell is a powerful strategy used by scientists to better understand the function of a protein of interest. Various inducible systems have been designed in eukaryotic cells to achieve this goal. Most of them rely on two distinct vectors, one encoding a protein that can regulate transcription by binding a compound X, and one hosting the cDNA encoding the protein of interest placed downstream of promoter sequences that can bind the protein regulated by compound X (e.g., tetracycline, ecdysone). The commercially available systems are not designed to allow cell- or tissue-specific regulated expression. Additionally, although these systems can be used to generate stable clones that can be induced to express a given protein, extensive screening is often required to eliminate the clones that display poor induction or high basal levels. In the present report, we aimed to design a pancreatic beta cell-specific tetracycline-inducible system. Since the classical two-vector based tetracycline-inducible system proved to be unsatisfactory in our hands, a single vector was eventually designed that allowed tight beta cell-specific tetracycline induction in unselected cell populations.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/46345
Statistics
Document views: 12 File downloads:
  • Full-text: 0