In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering.

Peloso A; Citro A; Corradetti V; Brambilla S; Oldani G; Calabrese F; Dominioni T; Maestri M; Cobianchi L

doi:10.1007/7651_2017_96

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Journal article

In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering.

Peloso A Department of Clinical, Surgical, Diagnostic and Paediatric Sciences, University of Pavia, Pavia, Italy. andreapeloso@hotmail.it.
Citro A San Raffaele Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Corradetti V IRCCS Policlinico San Matteo, Unit of Nephrology, Dialysis and Transplantation, Pavia, Italy.
Brambilla S University of Pavia, Pavia, Italy.
Oldani G Divisions of Abdominal and Transplantation Surgery, Hepato-Pancreato-Biliary Centre, Department of Surgery, Faculty of Medicine, University Hospital of Geneva, University of Geneva, Geneva, Switzerland.
Calabrese F Department of General Surgery, IRCCS Policlinico San Matteo, Pavia, Italy.
Dominioni T Department of General Surgery, IRCCS Policlinico San Matteo, Pavia, Italy.
Maestri M Department of General Surgery, IRCCS Policlinico San Matteo, Pavia, Italy.
Cobianchi L Department of Clinical, Surgical, Diagnostic and Paediatric Sciences, University of Pavia, Pavia, Italy.

2017-11-22

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Methods in molecular biology (Clifton, N.J.). - 2018

English Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition.

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