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Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells.
Journal article

Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells.

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  • 2003-05-03
Published in:
  • Molecular and cellular neurosciences. - 2003
Animals
Animals, Newborn
Brain
Cell Lineage
Dose-Response Relationship, Drug
Female
Fetus
Gene Expression Regulation
Genes, Reporter
Integrases
Lactation
Male
Mice
Mice, Transgenic
Mutagenesis
Oligodendroglia
Peripheral Nervous System
Protein Structure, Tertiary
Receptors, Estrogen
Recombinant Fusion Proteins
Schwann Cells
Stem Cells
Tamoxifen
Transgenes
Viral Proteins
English Inducible transgenesis provides a valuable technique for the analysis of gene function in vivo. We report the generation and characterization of mouse lines carrying glia lineage-specific transgenes expressing an improved variant of the tamoxifen-inducible Cre recombinase, CreERT2, where the recombinase is fused to a mutated ligand binding domain of the human estrogen receptor. Using a PLP-CreERT2 transgene, we have generated mice that show specific inducible Cre function, as analyzed by cross-breeding experiments into the Rosa26 Cre-LacZ reporter line, in developing and adult Schwann cells, in mature myelinating oligodendrocytes, and in undifferentiated NG2-positive oligodendrocyte precursors in the adult. Using a P0Cx-CreERT2 transgene, we have also established mouse lines with inducible Cre function specifically in the Schwann cell lineage. These tamoxifen-inducible CreERT2 lines will allow detailed spatiotemporally controlled analysis of gene functions in loxP-based conditional mutant mice in both developing and adult Schwann cells and in the oligodendrocyte lineage.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/47091
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