Deciphering enzymes. Genetic selection as a probe of structure and mechanism.

Woycechowsky KJ; Hilvert D

doi:10.1111/j.1432-1033.2004.04073.x

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Journal article

Deciphering enzymes. Genetic selection as a probe of structure and mechanism.

Woycechowsky KJ Laboratorium für Organische Chemie, Swiss Federal Institute of Technology, ETH-Hönggerberg, Zürich, Switzerland.
Hilvert D

2004-04-21

Published in:

European journal of biochemistry. - 2004

Structure-Activity Relationship

English The efficient engineering of enzymes with novel activities remains an ongoing challenge. Towards this end, genetic selection techniques provide a method for finding rare solutions to catalytic problems that requires only a limited foreknowledge of structure-function relationships. We have used genetic selections to extensively probe the structure and mechanism of chorismate mutases. The insights gained from these investigations will aid future enzyme design efforts.

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