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Genomic footprinting of the promoter regions of STE2 and STE3 genes in the yeast Saccharomyces cerevisiae.
Journal article

Genomic footprinting of the promoter regions of STE2 and STE3 genes in the yeast Saccharomyces cerevisiae.

  • 1993-12-20
Published in:
  • Journal of molecular biology. - 1993
Base Sequence
DNA Primers
Fungal Proteins
Gene Expression Regulation, Fungal
Genes, Fungal
Molecular Sequence Data
Nucleosomes
Promoter Regions, Genetic
RNA, Messenger
Receptors, Cell Surface
Receptors, G-Protein-Coupled
Receptors, Mating Factor
Receptors, Peptide
Receptors, Pheromone
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
TATA Box
Transcription Factors
English Dimethyl sulfate, DNase I and micrococcal nuclease DNA cleavage were combined with the ligation-mediated polymerase chain reaction to obtain high resolution maps of the promoter regions for two cell-type-specific genes: the a-specific STE2 gene and the alpha-specific STE3 gene. We find that MCM1 binds in vivo in a-cells to a 16 bp P-box sequence located in the STE2 UAS. In alpha-cells, the footprint pattern is extended relative to a-cells, consistent with the additional binding of MAT alpha 2 to the sequences flanking each end of the P-box. A nucleosome was found adjacent to the P-box of the transcriptionally repressed a-specific STE2 UAS in alpha-cells, positioned so that the nucleosome overlaps the TATA-box. In contrast, such well-positioned nucleosomes were not found for the transcriptionally active STE2 UAS in a-cells, where instead the TATA box appears to be bound to the general transcription factor TFIID. These observations support the hypothesis that MAT alpha 2 repression of a-specific genes is mediated by nucleosomes, perhaps by exclusion of TFIID from the TATA-box.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/47406
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