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Light-harvesting complex II in monocomponent and mixed lipid-protein monolayers.
Journal article

Light-harvesting complex II in monocomponent and mixed lipid-protein monolayers.

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  • 1998-09-12
Published in:
  • Biochimica et biophysica acta. - 1998
Chlorophyll
Light-Harvesting Protein Complexes
Lipid Metabolism
Liposomes
Membrane Proteins
Microscopy, Atomic Force
Phosphatidylcholines
Photosynthetic Reaction Center Complex Proteins
Photosystem II Protein Complex
Plant Leaves
Plant Proteins
Pressure
Protein Conformation
Secale
Spectrometry, Fluorescence
Spectrophotometry
Surface Properties
English Monomolecular layers at the air-water interface were formed directly with isolated largest light-harvesting pigment-protein complex of Photosystem II (LHC II) or out of egg yolk lecithin (EYL) liposomes containing incorporated LHC II. Pure protein monolayers showed a mean area of 1400 A2 per molecule at the air-water interface. Monolayers were deposited onto glass slides by means of Langmuir-Blodgett (LB) technique. Chlorophyll fluorescence of LHC II-LB and EYL-LHC II-LB films proved energetic coupling of chlorophyll a and b, thus indicating native conformation of LHC II within the monolayers. Scanning force microscopy (SFM) revealed ring-like structures formed in monocomponent protein layers as well as in mixed protein-lipid films. These results suggest that a structural arrangement of LHC II is favoured in a lipid environment but that the protein has itself a strong tendency for structural complex rearrangement in our system.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/61451
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