Microsatellite primers in the lichen symbiotic alga Trebouxia decolorans (Trebouxiophyceae).
- Dal Grande F Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, 60325 Frankfurt am Main, Germany.
- Beck A Department of Lichenology and Bryology, Botanische Staatssammlung München, 80638 Munich, Germany.
- Singh G Biodiversity and Conservation Biology, WSL Swiss Federal Research Institute, Zürcherstrasse 111, 8903 Birmensdorf, Switzerland.
- Schmitt I Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, 60325 Frankfurt am Main, Germany ; Institute of Ecology, Evolution and Diversity, Goethe Universität, Max-von-Laue-Str. 13, D-60438 Frankfurt, Germany.
- 2014-09-10
Published in:
- Applications in plant sciences. - 2013
English
UNLABELLED
PREMISE OF THE STUDY
Polymorphic microsatellite markers were developed for the symbiotic green alga Trebouxia decolorans to study fine-scale population structure and clonal diversity. •
METHODS AND RESULTS
Using Illumina pyrosequencing, 20 microsatellite primer sets were developed for T. decolorans. The primer sets were tested on 43 individuals sampled from four subpopulations in Germany. The primers amplified di-, tri-, and tetranucleotide repeats with three to 15 alleles per locus, and the unbiased haploid diversity per locus ranged from 0.636 to 0.821. •
CONCLUSIONS
The identified microsatellite markers will be useful to study the genetic diversity, dispersal, and reproductive mode of this common lichen photobiont.
PREMISE OF THE STUDY
Polymorphic microsatellite markers were developed for the symbiotic green alga Trebouxia decolorans to study fine-scale population structure and clonal diversity. •
METHODS AND RESULTS
Using Illumina pyrosequencing, 20 microsatellite primer sets were developed for T. decolorans. The primer sets were tested on 43 individuals sampled from four subpopulations in Germany. The primers amplified di-, tri-, and tetranucleotide repeats with three to 15 alleles per locus, and the unbiased haploid diversity per locus ranged from 0.636 to 0.821. •
CONCLUSIONS
The identified microsatellite markers will be useful to study the genetic diversity, dispersal, and reproductive mode of this common lichen photobiont.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.3732/apps.1200400
- PMID 25202529
- Persistent URL
- https://sonar.ch/global/documents/62470
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