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Functionally distinct nucleosome-free regions in yeast require Rad7 and Rad16 for nucleotide excision repair.
Journal article

Functionally distinct nucleosome-free regions in yeast require Rad7 and Rad16 for nucleotide excision repair.

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  • 2008-03-11
Published in:
  • DNA repair. - 2008
Adenosine Triphosphatases
DNA Repair
DNA, Fungal
DNA-Binding Proteins
Fungal Proteins
Mutation
Nucleosomes
Pyrimidine Dimers
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors
English In yeast, Rad7 and Rad16 are two proteins required for nucleotide excision repair (NER) of non-transcribed chromatin. They have roles in damage recognition, in the postincision steps of NER, and in ultraviolet-light-dependent histone H3 acetylation. Moreover, Rad16 is an ATP-ase of the SNF2 superfamily and therefore might facilitate chromatin repair by nucleosome remodelling. Here, we used yeast rad7 Delta rad16 Delta mutants and show that Rad7-Rad16 is also required for NER of UV-lesions in three functionally distinct nucleosome-free regions (NFRs), the promoter and 3'-end of the URA3 gene and the ARS1 origin of replication. Moreover, rapid repair of UV-lesions by photolyase confirmed that nucleosomes were absent and that neither UV-damage formation nor rad7 Delta rad16 Delta mutations altered chromatin accessibility in NFRs. The data are consistent with a role of Rad7-Rad16 in damage recognition and processing in absence of nucleosomes. An additional role in nucleosome remodelling is discussed.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/67195
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