Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae.

Kuratli S; Hemphill A; Lindh J; Smith DF; Connolly B

doi:10.1016/s0166-6851(01)00287-0

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Journal article

Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae.

Kuratli S Institute of Parasitology, University of Bern, CH-3012, Bern, Switzerland.
Hemphill A
Lindh J
Smith DF
Connolly B

2001-06-23

Published in:

Molecular and biochemical parasitology. - 2001

Fluorescent Antibody Technique

English The Trichinella tsJ5 gene is preferentially expressed in muscle larvae of Trichinella spiralis and encodes a novel protein. Previous observations have shown tsJ5 to be expressed at higher levels in encapsulating species than in non-encapsulating species and down-regulation of gene expression in T. pseudospiralis to be correlated with a lower protein abundance in the muscle larva of this species. In the present study we have determined the full-length cDNA sequence of the tsJ5 homologue in T. pseudospiralis (tpJ5). Antigens recognised by an anti-J5 antibody are found on the cuticular surface of both T. spiralis and T. pseudospiralis muscle larvae, as well as in the body wall muscle. We show that both the TSJ5 and TPJ5 proteins are found in the excretory/secretory fractions collected from muscle larva cultured in vitro and that despite the absence of a typical N-terminal signal sequence, secretion of pTSJ5 is mediated through the classical ER/Golgi secretory pathway.

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