Towards amperometric immunosensor devices.

Tiefenauer LX; Kossek S; Padeste C; Thiébaud P

doi:10.1016/s0956-5663(97)85339-0

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Journal article

Towards amperometric immunosensor devices.

Tiefenauer LX Paul Scherrer Institut, Micro- and Nanostructures Laboratory, Villigen, Switzerland. tiefenauer@psi.ch
Kossek S
Padeste C
Thiébaud P

1997-01-01

Published in:

Biosensors & bioelectronics. - 1997

Enzyme-Linked Immunosorbent Assay

English In contrast to optical immunosensors, the electrochemical detection of an immunanalytical reaction does require a labeling, but allows an easier discrimination of specific and non-specific binding. We present a concept and first results for a multivalent amperometric immunosensor system which is based on silicon technology. The capture molecule streptavidin, covalently immobilized on silica, allows the immobilization of biotinylated antigens at a defined density. A nanostructured gold electrode serving as a stable network of nanowires is expected to be beneficial for the electrochemical detection of bound ferrocene-labeled antibody molecules. The results presented focus on site-specific immobilization of streptavidin on silica and reduction of non-specific binding of proteins.

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