Changes to the Aqueous Humor Proteome during Glaucoma.
- Kaeslin MA Center of Laboratory Medicine, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
- Killer HE Department of Ophthalmology, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
- Fuhrer CA Center of Laboratory Medicine, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
- Zeleny N Department of Ophthalmology, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
- Huber AR Center of Laboratory Medicine, Cantonal Hospital Aarau, Tellstrasse 25, 5001 Aarau, Switzerland.
- Neutzner A Department of Biomedicine and Department of Ophthalmology, University Basel, Basel, Switzerland.
- 2016-10-28
Published in:
- PloS one. - 2016
Aged
Aged, 80 and over
Aqueous Humor
Case-Control Studies
Female
Glaucoma
Humans
Male
Middle Aged
Proteome
Up-Regulation
English
PURPOSE
To investigate the aqueous humor proteome in patients with glaucoma and a control group.
METHOD
Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueous humor by hyper reaction monitoring mass spectrometry (HRM-MS) based on SWATH technology was performed.
RESULTS
Expression levels of 87 proteins were found to be different between glaucomatous and control aqueous humor. Of the 87 proteins, 34 were significantly upregulated, whereas 53 proteins were downregulated in the aqueous humor from glaucoma patients compared to controls. Differentially expressed proteins were found to be involved in cholesterol-related, inflammatory, metabolic, antioxidant as well as proteolysis-related processes.
CONCLUSION
Glaucoma leads to profound changes to the aqueous humor proteome consistent with an altered metabolic state, an inflammatory response and impaired antioxidant defense.
To investigate the aqueous humor proteome in patients with glaucoma and a control group.
METHOD
Aqueous humor was obtained from five human donors diagnosed with primary open angle glaucoma (POAG) and five age- and sex-matched controls undergoing cataract surgery. Quantitative proteome analysis of the aqueous humor by hyper reaction monitoring mass spectrometry (HRM-MS) based on SWATH technology was performed.
RESULTS
Expression levels of 87 proteins were found to be different between glaucomatous and control aqueous humor. Of the 87 proteins, 34 were significantly upregulated, whereas 53 proteins were downregulated in the aqueous humor from glaucoma patients compared to controls. Differentially expressed proteins were found to be involved in cholesterol-related, inflammatory, metabolic, antioxidant as well as proteolysis-related processes.
CONCLUSION
Glaucoma leads to profound changes to the aqueous humor proteome consistent with an altered metabolic state, an inflammatory response and impaired antioxidant defense.
- Language
-
- English
- Open access status
- gold
- Identifiers
-
- DOI 10.1371/journal.pone.0165314
- PMID 27788204
- Persistent URL
- https://sonar.ch/global/documents/84117
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