Journal article
Altered expression and localization of the tight junction protein ZO-1 in primary and metastatic pancreatic cancer.
- Kleeff J Department of Visceral and Transplantation Surgery, University of Bern, Switzerland.
- Shi X
- Bode HP
- Hoover K
- Shrikhande S
- Bryant PJ
- Korc M
- Büchler MW
- Friess H
- 2001-10-09
Published in:
- Pancreas. - 2001
Adult
Aged
Alternative Splicing
Blotting, Northern
Blotting, Southern
Blotting, Western
Carcinoma, Pancreatic Ductal
Chronic Disease
Female
Gene Expression
Humans
Lymphatic Metastasis
Male
Membrane Proteins
Microscopy, Confocal
Middle Aged
Neoplasm Metastasis
Pancreas
Pancreatic Neoplasms
Pancreatitis
Phosphoproteins
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Zonula Occludens-1 Protein
English
INTRODUCTION
ZO-1 is a tight junction membrane protein that plays a critical role in cell-cell interaction, proliferation, and differ entiation.
AIM
To localize and evaluate the expression of ZO-1 in the normal human pancreas, in pancreatic ductal adenocarcinoma (PDAC), and in chronic pancreatitis (CP).
METHODOLOGY AND RESULTS
Northern and Western blot analysis revealed ZO-1 expression in all six tested pancreatic cancer cell lines. Expression of ZO-1 mRNA was increased sixfold in PDAC samples in comparison with normal samples (p = 0.04). Confocal microscopy revealed the presence of ZO-1 in the apical and apicolateral areas of ductular cells in the normal pancreas. Similarly, in CP, ZO-1 was localized at apical and apicolateral areas of small proliferating ductular cells and large metaplastic ducts. In PDAC, however, ZO-1 expression was observed irrespective of whether the cancer cells formed duct-like structures or exhibited a diffuse infiltrating pattern. Metastatic pancreatic cancer cells within lymph nodes displayed variable staining patterns, ranging from apical and apicolateral to a diffuse membranous staining.
CONCLUSION
These observations suggest that ZO-1 is overexpressed in PDAC and raise the possibility that this overexpression may confer a metastatic advantage to pancreatic cancer cells.
ZO-1 is a tight junction membrane protein that plays a critical role in cell-cell interaction, proliferation, and differ entiation.
AIM
To localize and evaluate the expression of ZO-1 in the normal human pancreas, in pancreatic ductal adenocarcinoma (PDAC), and in chronic pancreatitis (CP).
METHODOLOGY AND RESULTS
Northern and Western blot analysis revealed ZO-1 expression in all six tested pancreatic cancer cell lines. Expression of ZO-1 mRNA was increased sixfold in PDAC samples in comparison with normal samples (p = 0.04). Confocal microscopy revealed the presence of ZO-1 in the apical and apicolateral areas of ductular cells in the normal pancreas. Similarly, in CP, ZO-1 was localized at apical and apicolateral areas of small proliferating ductular cells and large metaplastic ducts. In PDAC, however, ZO-1 expression was observed irrespective of whether the cancer cells formed duct-like structures or exhibited a diffuse infiltrating pattern. Metastatic pancreatic cancer cells within lymph nodes displayed variable staining patterns, ranging from apical and apicolateral to a diffuse membranous staining.
CONCLUSION
These observations suggest that ZO-1 is overexpressed in PDAC and raise the possibility that this overexpression may confer a metastatic advantage to pancreatic cancer cells.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1097/00006676-200110000-00006
- PMID 11590321
- Persistent URL
- https://sonar.ch/global/documents/875
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