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B-cell isotype control in atopy and asthma assessed with cDNA array technology.
Journal article

B-cell isotype control in atopy and asthma assessed with cDNA array technology.

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  • 2001-03-10
Published in:
  • American journal of physiology. Lung cellular and molecular physiology. - 2001
Adolescent
Adult
Aged
Asthma
B-Lymphocytes
Cell Separation
DNA, Complementary
Female
Flow Cytometry
Gene Expression
Humans
Hypersensitivity
Immunoglobulin E
Immunoglobulin Isotypes
Male
Middle Aged
Oligonucleotide Array Sequence Analysis
Receptors, Interleukin-2
Reference Values
Severity of Illness Index
English B-cell isotype switching and the production of IgE is regulated by a variety of gene products through different mechanisms. A better understanding of these processes has the potential to identify markers of disease and new therapeutic targets. The aim of the study was to investigate human B-cell isotype control and IgE production in atopy and asthma with cDNA array technology. Eighteen atopic asthmatic, eight atopic nonasthmatic, and fourteen healthy control subjects were included. Peripheral blood mononuclear cells were separated by gradient centrifugation, mRNA was purified, and the reverse-transcribed probes were hybridized to cDNA membranes. Group differences were assessed with the Mann-Whitney U-test. Twenty-three of seventy-eight tested IgE-related genes had significantly altered expression in atopy and asthma compared with that in the healthy subjects. The differentially expressed genes include surface molecules involved in T- and B-cell interaction and activation, cytokines, intracellular signaling products, and transcription factors. In conclusion, both atopic nonasthmatic and atopic asthmatic individuals had activated proinflammatory pathways, a minimal requirement for B-cell isotype switching, and a clear net pro-IgE cytokine climate.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/92786
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