Journal article
Improved Adipocyte Viability in Autologous Fat Grafting With Ascorbic Acid-Supplemented Tumescent Solution.
- Lunger A From the Department of Plastic, Reconstructive, Aesthetic and Hand Surgery.
- Ismail T From the Department of Plastic, Reconstructive, Aesthetic and Hand Surgery.
- Todorov A Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel.
- Buergin J From the Department of Plastic, Reconstructive, Aesthetic and Hand Surgery.
- Lunger F Department of General Surgery, Cantonal Hospital of Winterthur, Winterthur, Switzerland.
- Oberhauser I From the Department of Plastic, Reconstructive, Aesthetic and Hand Surgery.
- Haug M From the Department of Plastic, Reconstructive, Aesthetic and Hand Surgery.
- Kalbermatten DF From the Department of Plastic, Reconstructive, Aesthetic and Hand Surgery.
- Largo RD Department of Plastic Surgery, The University of Texas, MD Anderson Cancer Center, Houston, TX.
- Martin I Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel.
- Scherberich A Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel.
- Schaefer DJ From the Department of Plastic, Reconstructive, Aesthetic and Hand Surgery.
- 2019-09-17
Published in:
- Annals of plastic surgery. - 2019
Abdominal Fat
Adipocytes
Adipose Tissue
Adult
Aged
Anesthetics, Local
Ascorbic Acid
Cell Differentiation
Cell Survival
Cohort Studies
Female
Graft Survival
Humans
Lipectomy
Middle Aged
Sensitivity and Specificity
Stromal Cells
Transplantation, Autologous
English
INTRODUCTION
In reconstructive surgery, fat volume augmentation is often necessary for esthetic or functional reasons. As an alternative to synthetic and xenogeneic materials, autologous fat grafting (AFG) based on liposuction is gaining popularity, yet successful transplantation and long-term volume maintenance are difficult. Standard tumescent solution formulations neglect adipocyte and stromal vascular fraction (SVF) cell survival during extraction, as well as SVF differentiation into adipocytes thereafter, all of which are crucial for the success of AFG. Here we hypothesized that addition of ascorbic acid (AA) to the tumescent solution could prevent liposuction-induced cell damage.
MATERIALS AND METHODS
The effect of 0.1 mmol/L AA in tumescent solution was investigated in a previously described ex vivo model of AFG. Briefly, excision fat was infiltrated with tumescent solution, with or without AA, and incubated for 20 minutes at 37°C. Hand-assisted liposuction was then performed with a blunt cannula. Total cell viability, clonogenicity, and differentiation capacity of the SVF cells were assessed.
RESULTS
With AA, 10.3% more cells and in particular 14.9% more adipocytes survived liposuction. Clonogenicity, adipocyte and osteoblast differentiation by SVF cells remained unchanged.
CONCLUSIONS
Addition of AA successfully improved survival of adipocytes during liposuction without affecting SVF growth and differentiation. This study therefore identified a useful supplement to the tumescent solution which may lead to improving AFG success.
In reconstructive surgery, fat volume augmentation is often necessary for esthetic or functional reasons. As an alternative to synthetic and xenogeneic materials, autologous fat grafting (AFG) based on liposuction is gaining popularity, yet successful transplantation and long-term volume maintenance are difficult. Standard tumescent solution formulations neglect adipocyte and stromal vascular fraction (SVF) cell survival during extraction, as well as SVF differentiation into adipocytes thereafter, all of which are crucial for the success of AFG. Here we hypothesized that addition of ascorbic acid (AA) to the tumescent solution could prevent liposuction-induced cell damage.
MATERIALS AND METHODS
The effect of 0.1 mmol/L AA in tumescent solution was investigated in a previously described ex vivo model of AFG. Briefly, excision fat was infiltrated with tumescent solution, with or without AA, and incubated for 20 minutes at 37°C. Hand-assisted liposuction was then performed with a blunt cannula. Total cell viability, clonogenicity, and differentiation capacity of the SVF cells were assessed.
RESULTS
With AA, 10.3% more cells and in particular 14.9% more adipocytes survived liposuction. Clonogenicity, adipocyte and osteoblast differentiation by SVF cells remained unchanged.
CONCLUSIONS
Addition of AA successfully improved survival of adipocytes during liposuction without affecting SVF growth and differentiation. This study therefore identified a useful supplement to the tumescent solution which may lead to improving AFG success.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1097/SAP.0000000000001857
- PMID 31524744
- Persistent URL
- https://sonar.ch/global/documents/93591
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