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Site-Specific Polymer Conjugation Stabilizes Therapeutic Enzymes in the Gastrointestinal Tract.
Journal article

Site-Specific Polymer Conjugation Stabilizes Therapeutic Enzymes in the Gastrointestinal Tract.

  • Schulz JD Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zurich (ETH Zurich), 8093, Zurich, Switzerland.
  • Patt M Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zurich (ETH Zurich), 8093, Zurich, Switzerland.
  • Basler S Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zurich (ETH Zurich), 8093, Zurich, Switzerland.
  • Kries H Department of Chemistry and Applied Biosciences, Laboratory of Organic Chemistry, Swiss Federal Institute of Technology Zurich (ETH Zurich), 8093, Zurich, Switzerland.
  • Hilvert D Department of Chemistry and Applied Biosciences, Laboratory of Organic Chemistry, Swiss Federal Institute of Technology Zurich (ETH Zurich), 8093, Zurich, Switzerland.
  • Gauthier MA EMT Research Center, Institut National de la Recherche Scientifique (INRS), J3X 1S2 Varennes, Quebec, Canada.
  • Leroux JC Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology Zurich (ETH Zurich), 8093, Zurich, Switzerland.
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  • 2015-12-08
Published in:
  • Advanced materials (Deerfield Beach, Fla.). - 2016
gastrointestinal stability
poly(ethylene glycol)
site-specific polymer conjugation, therapeutic enzymes
Animals
Binding Sites
Catalytic Domain
Endopeptidases
Enzyme Stability
Gastrointestinal Tract
Models, Molecular
Myxococcus xanthus
Polyethylene Glycols
Rats
English The site-specific conjugation of polymers to multiple engineered cysteine residues of a prolyl endopeptidase leads to its stabilization in the gastrointestinal tract of rats, without compromising the activity relative to the native enzyme. The importance of polymer attachment sites is investigated, as well as the significance of polymer structure.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/231555
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