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Journal article

Increased Proangiogenic Activity of Mobilized CD34+ Progenitor Cells of Patients With Acute ST-Segment-Elevation Myocardial Infarction: Role of Differential MicroRNA-378 Expression.

  • Templin C From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.). Christian.Templin@usz.ch.
  • Volkmann J From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Emmert MY From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Mocharla P From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Müller M From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Kraenkel N From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Ghadri JR From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Meyer M From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Styp-Rekowska B From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Briand S From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Klingenberg R From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Jaguszewski M From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Matter CM From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Djonov V From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Mach F From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Windecker S From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Hoerstrup SP From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Thum T From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Lüscher TF From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
  • Landmesser U From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
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  • 2017-01-08
Published in:
  • Arteriosclerosis, thrombosis, and vascular biology. - 2017
English OBJECTIVE
Proangiogenic effects of mobilized bone marrow-derived stem/progenitor cells are essential for cardiac repair after myocardial infarction. MicroRNAs (miRNA/miR) are key regulators of angiogenesis. We investigated the differential regulation of angio-miRs, that is, miRNAs regulating neovascularization, in mobilized CD34+ progenitor cells obtained from patients with an acute ST-segment-elevation myocardial infarction (STEMI) as compared with those with stable coronary artery disease or healthy subjects.


APPROACH AND RESULTS
CD34+ progenitor cells were isolated from patients with STEMI (on day 0 and day 5), stable coronary artery disease, and healthy subjects (n=27). CD34+ progenitor cells of patients with STEMI exhibited increased proangiogenic activity as compared with CD34+ cells from the other groups. Using a polymerase chain reaction-based miRNA-array and real-time polymerase chain reaction validation, we identified a profound upregulation of 2 known angio-miRs, that are, miR-378 and let-7b, in CD34+ cells of patients with STEMI. Especially, we demonstrate that miR-378 is a critical regulator of the proangiogenic capacity of CD34+ progenitor cells and its stimulatory effects on endothelial cells in vitro and in vivo, whereas let-7b upregulation in CD34+ cells failed to proof its effect on endothelial cells in vivo.


CONCLUSIONS
The present study demonstrates a significant upregulation of the angio-miRs miR-378 and let-7b in mobilized CD34+ progenitor cells of patients with STEMI. The increased proangiogenic activity of these cells in patients with STEMI and the observation that in particular miR-378 regulates the angiogenic capacity of CD34+ progenitor cells in vivo suggest that this unique miRNA expression pattern represents a novel endogenous repair mechanism activated in acute myocardial infarction.
Language
  • English
Open access status
bronze
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Persistent URL
https://sonar.ch/global/documents/278669
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