Journal article
Circulating CD14+ and CD14highCD16- classical monocytes are reduced in patients with signs of plaque neovascularization in the carotid artery.
- Ammirati E Cardiothoracic Department, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy; De Gasperis Cardio Center, Niguarda Ca' Granda Hospital, Milan, Italy. Electronic address: ammirati.enrico@hsr.it.
- Moroni F Cardiothoracic Department, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy. Electronic address: moroni@studenti.unisr.it.
- Magnoni M Cardiothoracic Department, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy.
- Di Terlizzi S FRACTAL - Flow cytometry Resource Advanced Cytometry Technical Applications Laboratory, San Raffaele Scientific Institute, Milan, Italy.
- Villa C FRACTAL - Flow cytometry Resource Advanced Cytometry Technical Applications Laboratory, San Raffaele Scientific Institute, Milan, Italy.
- Sizzano F Nestlé Institute of Health Sciences, Biobanking & Flow Cytometry Core EPFL, Innovation Park Bâtiment H, Lausanne, Switzerland.
- Palini A Nestlé Institute of Health Sciences, Biobanking & Flow Cytometry Core EPFL, Innovation Park Bâtiment H, Lausanne, Switzerland.
- Garlaschelli K Center SISA for the Study of Atherosclerosis, Bassini Hospital, Cinisello Balsamo, Italy.
- Tripiciano F Hematology and Blood Transfusion Service, San Raffaele Scientific Institute, Milan, Italy.
- Scotti I Department of Rheumatology, Istituto Ortopedico Gaetano Pini, Milan, Italy.
- Catapano AL IRCCS - Multimedica Hospital, Sesto San Giovanni, Italy; Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy.
- Manfredi AA Unit of Internal Medicine & Clinical Immunology, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy.
- Norata GD Center SISA for the Study of Atherosclerosis, Bassini Hospital, Cinisello Balsamo, Italy; Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia.
- Camici PG Cardiothoracic Department, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy.
- 2016-10-19
Published in:
- Atherosclerosis. - 2016
Carotid atherosclerosis
Classical monocytes
Contrast enhanced ultrasound
Monocytes subsets
Plaque neovascularization
Aged
Biomarkers
C-Reactive Protein
Carotid Arteries
Carotid Stenosis
Contrast Media
Female
Flow Cytometry
Humans
Inflammation Mediators
Interleukin-6
Lipopolysaccharide Receptors
Male
Middle Aged
Monocytes
Neovascularization, Pathologic
Phospholipids
Plaque, Atherosclerotic
Receptors, IgG
Severity of Illness Index
Sulfur Hexafluoride
Ultrasonography, Doppler, Duplex
English
BACKGROUND AND AIMS
Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking.
METHODS
Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry.
RESULTS
No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029).
CONCLUSIONS
Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored.
Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking.
METHODS
Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry.
RESULTS
No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029).
CONCLUSIONS
Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.atherosclerosis.2016.10.004
- PMID 27751505
- Persistent URL
- https://sonar.ch/global/documents/97022
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